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multiple human adult brain tissue northern blots  (TaKaRa)


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    TaKaRa multiple human adult brain tissue northern blots
    Multiple Human Adult Brain Tissue Northern Blots, supplied by TaKaRa, used in various techniques. Bioz Stars score: 94/100, based on 1153 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+multiple+tissue+northern+blots/Human+Brain+Total+RNA/us11932908-1087-19-31
    Average 94 stars, based on 1153 article reviews
    multiple human adult brain tissue northern blots - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Northern Blot:

    Article Title: Antibodies that inhibit TSLP activity
    Article Snippet: .. An aliquot of a radiolabeled probe was added to two different human multiple tissue Northern blots (Clontech, Palo Alto, Calif.; Biochain, Palo Alto, Calif.). ..

    Article Title: Diagnosis of hereditary spastic paraplegias (HSP) by detection of a mutation in the KIAA1840 gene or protein
    Article Snippet: The corresponding cDNAs were synthesized using random hexamers in the presence of Thermoscript reverse transcriptase as recommended by the supplier (Invitrogen). .. Human multiple tissue northern blots (Clontech) were hybridized at 68° C. for 1 hour with a mix of these probes aP32-labeled by random priming (Prime-it II Random Primer Labeling kit, Stratagene) and purified using ProbeQuant G-50 micro columns (Amersham Biosciences) in accordance to the manufacturer's recommendations to reach a specific activity of at least 1×109 cpm/μg. .. Membranes were then washed as recommended by Clontech then exposed to X-Ray film for autoradiography.

    Article Title: Overexpression of C16orf74 is involved in aggressive pancreatic cancers
    Article Snippet: Normal tissue sections were purchased from Biochain (Hayward, CA, USA). .. Human multiple-tissue Northern blots (Clontech, Palo Alto, CA) or a membrane including RNA samples from cancer cell lines and normal organs (Clontech) were hybridized with a [ 32 P]-dCTP-labeled amplification product of C16orf74 prepared by RT-PCR (see below). ..

    Article Title: Systematic identification of regulatory proteins critical for T-cell activation
    Article Snippet: .. Human Multiple Tissue Northern Blots were purchased from Clontech. ..

    Article Title: BAFF, inhibitors thereof and their use in the modulation of B-cell response and treatment of autoimmune disorders
    Article Snippet: Human B cells of >97% purity were isolated from peripheral blood or umbilical cord blood using anti-CD19 magnetic beads (M450, Dynal, Oslo, Norway) as described (13). .. Northern Blot Analysis Northern blot analysis was carried out using Human Multiple Tissue Northern Blots I and II (Clontech #7760-1 and #7759-1). ..

    Article Title: Involvement of maternal embryonic leucine zipper kinase (MELK) in mammary carcinogenesis through interaction with Bcl-G, a pro-apoptotic member of the Bcl-2 family
    Article Snippet: One microgram of each mRNA, along with polyA(+) RNAs isolated from normal mammary gland, lung, heart, liver, kidney and brain (Takara Clontech), were separated on 1% denaturing agarose gels and transferred to nylon membranes (breast cancer Northern blots). .. Breast cancer Northern blots and human multiple-tissue Northern blots (Takara Clontech) were hybridized with [α 32 P]-dCTP-labeled PCR products of MELK cDNA prepared by RT-PCR (see below). ..

    Article Title: Compositions and methods for modulating lymphocyte activity
    Article Snippet: .. Mouse and human multiple tissue northern blots (Clontech) were probed with cDNA fragments labeled by 32P-dCTP with Random Primed DNA Labeling kit (Roche). ..

    Article Title: Diagnosis of hereditary spastic paraplegias (HSP) by detection of a mutation in the KIAA1840 gene or protein
    Article Snippet: The corresponding cDNAs were synthesized using random hexamers in the presence of Thermoscript reverse transcriptase as recommended by the supplier (Invitrogen). .. Human multiple tissue northern blots (Clontech) were hybridized at 68° C. for 1 hour with a mix of these probes aP32-labeled by random priming (Prime-it II Random Primer Labeling kit, Stratagene) and purified using ProbeQuant G-50 micro columns (Amersham Biosciences) in accordance to the manufacturer's recommendations to reach a specific activity of at least 1×109 cpm/μg. .. Membranes were then washed as recommended by Clontech then exposed to X-Ray film for autoradiography.

    Labeling:

    Article Title: Diagnosis of hereditary spastic paraplegias (HSP) by detection of a mutation in the KIAA1840 gene or protein
    Article Snippet: The corresponding cDNAs were synthesized using random hexamers in the presence of Thermoscript reverse transcriptase as recommended by the supplier (Invitrogen). .. Human multiple tissue northern blots (Clontech) were hybridized at 68° C. for 1 hour with a mix of these probes aP32-labeled by random priming (Prime-it II Random Primer Labeling kit, Stratagene) and purified using ProbeQuant G-50 micro columns (Amersham Biosciences) in accordance to the manufacturer's recommendations to reach a specific activity of at least 1×109 cpm/μg. .. Membranes were then washed as recommended by Clontech then exposed to X-Ray film for autoradiography.

    Article Title: Compositions and methods for modulating lymphocyte activity
    Article Snippet: .. Mouse and human multiple tissue northern blots (Clontech) were probed with cDNA fragments labeled by 32P-dCTP with Random Primed DNA Labeling kit (Roche). ..

    Article Title: Diagnosis of hereditary spastic paraplegias (HSP) by detection of a mutation in the KIAA1840 gene or protein
    Article Snippet: The corresponding cDNAs were synthesized using random hexamers in the presence of Thermoscript reverse transcriptase as recommended by the supplier (Invitrogen). .. Human multiple tissue northern blots (Clontech) were hybridized at 68° C. for 1 hour with a mix of these probes aP32-labeled by random priming (Prime-it II Random Primer Labeling kit, Stratagene) and purified using ProbeQuant G-50 micro columns (Amersham Biosciences) in accordance to the manufacturer's recommendations to reach a specific activity of at least 1×109 cpm/μg. .. Membranes were then washed as recommended by Clontech then exposed to X-Ray film for autoradiography.

    Purification:

    Article Title: Diagnosis of hereditary spastic paraplegias (HSP) by detection of a mutation in the KIAA1840 gene or protein
    Article Snippet: The corresponding cDNAs were synthesized using random hexamers in the presence of Thermoscript reverse transcriptase as recommended by the supplier (Invitrogen). .. Human multiple tissue northern blots (Clontech) were hybridized at 68° C. for 1 hour with a mix of these probes aP32-labeled by random priming (Prime-it II Random Primer Labeling kit, Stratagene) and purified using ProbeQuant G-50 micro columns (Amersham Biosciences) in accordance to the manufacturer's recommendations to reach a specific activity of at least 1×109 cpm/μg. .. Membranes were then washed as recommended by Clontech then exposed to X-Ray film for autoradiography.

    Article Title: Diagnosis of hereditary spastic paraplegias (HSP) by detection of a mutation in the KIAA1840 gene or protein
    Article Snippet: The corresponding cDNAs were synthesized using random hexamers in the presence of Thermoscript reverse transcriptase as recommended by the supplier (Invitrogen). .. Human multiple tissue northern blots (Clontech) were hybridized at 68° C. for 1 hour with a mix of these probes aP32-labeled by random priming (Prime-it II Random Primer Labeling kit, Stratagene) and purified using ProbeQuant G-50 micro columns (Amersham Biosciences) in accordance to the manufacturer's recommendations to reach a specific activity of at least 1×109 cpm/μg. .. Membranes were then washed as recommended by Clontech then exposed to X-Ray film for autoradiography.

    Activity Assay:

    Article Title: Diagnosis of hereditary spastic paraplegias (HSP) by detection of a mutation in the KIAA1840 gene or protein
    Article Snippet: The corresponding cDNAs were synthesized using random hexamers in the presence of Thermoscript reverse transcriptase as recommended by the supplier (Invitrogen). .. Human multiple tissue northern blots (Clontech) were hybridized at 68° C. for 1 hour with a mix of these probes aP32-labeled by random priming (Prime-it II Random Primer Labeling kit, Stratagene) and purified using ProbeQuant G-50 micro columns (Amersham Biosciences) in accordance to the manufacturer's recommendations to reach a specific activity of at least 1×109 cpm/μg. .. Membranes were then washed as recommended by Clontech then exposed to X-Ray film for autoradiography.

    Article Title: Diagnosis of hereditary spastic paraplegias (HSP) by detection of a mutation in the KIAA1840 gene or protein
    Article Snippet: The corresponding cDNAs were synthesized using random hexamers in the presence of Thermoscript reverse transcriptase as recommended by the supplier (Invitrogen). .. Human multiple tissue northern blots (Clontech) were hybridized at 68° C. for 1 hour with a mix of these probes aP32-labeled by random priming (Prime-it II Random Primer Labeling kit, Stratagene) and purified using ProbeQuant G-50 micro columns (Amersham Biosciences) in accordance to the manufacturer's recommendations to reach a specific activity of at least 1×109 cpm/μg. .. Membranes were then washed as recommended by Clontech then exposed to X-Ray film for autoradiography.

    Membrane:

    Article Title: Overexpression of C16orf74 is involved in aggressive pancreatic cancers
    Article Snippet: Normal tissue sections were purchased from Biochain (Hayward, CA, USA). .. Human multiple-tissue Northern blots (Clontech, Palo Alto, CA) or a membrane including RNA samples from cancer cell lines and normal organs (Clontech) were hybridized with a [ 32 P]-dCTP-labeled amplification product of C16orf74 prepared by RT-PCR (see below). ..

    Amplification:

    Article Title: Overexpression of C16orf74 is involved in aggressive pancreatic cancers
    Article Snippet: Normal tissue sections were purchased from Biochain (Hayward, CA, USA). .. Human multiple-tissue Northern blots (Clontech, Palo Alto, CA) or a membrane including RNA samples from cancer cell lines and normal organs (Clontech) were hybridized with a [ 32 P]-dCTP-labeled amplification product of C16orf74 prepared by RT-PCR (see below). ..

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Overexpression of C16orf74 is involved in aggressive pancreatic cancers
    Article Snippet: Normal tissue sections were purchased from Biochain (Hayward, CA, USA). .. Human multiple-tissue Northern blots (Clontech, Palo Alto, CA) or a membrane including RNA samples from cancer cell lines and normal organs (Clontech) were hybridized with a [ 32 P]-dCTP-labeled amplification product of C16orf74 prepared by RT-PCR (see below). ..

    Article Title: Involvement of maternal embryonic leucine zipper kinase (MELK) in mammary carcinogenesis through interaction with Bcl-G, a pro-apoptotic member of the Bcl-2 family
    Article Snippet: One microgram of each mRNA, along with polyA(+) RNAs isolated from normal mammary gland, lung, heart, liver, kidney and brain (Takara Clontech), were separated on 1% denaturing agarose gels and transferred to nylon membranes (breast cancer Northern blots). .. Breast cancer Northern blots and human multiple-tissue Northern blots (Takara Clontech) were hybridized with [α 32 P]-dCTP-labeled PCR products of MELK cDNA prepared by RT-PCR (see below). ..

    Polymerase Chain Reaction:

    Article Title: Involvement of maternal embryonic leucine zipper kinase (MELK) in mammary carcinogenesis through interaction with Bcl-G, a pro-apoptotic member of the Bcl-2 family
    Article Snippet: One microgram of each mRNA, along with polyA(+) RNAs isolated from normal mammary gland, lung, heart, liver, kidney and brain (Takara Clontech), were separated on 1% denaturing agarose gels and transferred to nylon membranes (breast cancer Northern blots). .. Breast cancer Northern blots and human multiple-tissue Northern blots (Takara Clontech) were hybridized with [α 32 P]-dCTP-labeled PCR products of MELK cDNA prepared by RT-PCR (see below). ..

    Random Primed:

    Article Title: Compositions and methods for modulating lymphocyte activity
    Article Snippet: .. Mouse and human multiple tissue northern blots (Clontech) were probed with cDNA fragments labeled by 32P-dCTP with Random Primed DNA Labeling kit (Roche). ..

    DNA Labeling:

    Article Title: Compositions and methods for modulating lymphocyte activity
    Article Snippet: .. Mouse and human multiple tissue northern blots (Clontech) were probed with cDNA fragments labeled by 32P-dCTP with Random Primed DNA Labeling kit (Roche). ..



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    Expression and distribution of MELK in <t>human</t> normal tissues and breast cancer cell lines. (a) Expression of MELK in 12 breast cancer specimens (case number; 42, 102, 247, 252, 302, 473, 478, 502, 552, 646, 769 and 779) by semi-quantitative RT-PCR. GAPDH served as a quantitative internal control. (b) <t>Multiple</t> <t>tissue</t> <t>Northern</t> blot analysis demonstrated that an approximately 2.7 kb MELK transcript was detected in the testis, thymus and small intestine. PBL, peripheral blood leukocytes. (c) Breast cancer cell line Northern blot analysis revealed that approximately 2.4 to 2.7 kb MELK variants were specifically expressed in breast cancer cell lines, but not in normal vital organs. (d) Schematic representation of three variant transcripts identified by cDNA library screening (see Materials and methods). White boxes indicate a coding region and black boxes indicate a non-coding region. Black and grey triangles indicate initiation codons, and white triangles indicate stop codons. Exon numbers are shown above each box. (e) In vitro translation assay of each variant isolated from cDNA library screening. The number within parentheses represents the predicted molecular weight (kDa) of each variant protein. (f) Expression of MELK proteins in eight breast cancer cell lines as well as human mammary epithelial cells (HMECs) shown by western blot analysis with an anti-MELK antibody. β-Actin served as a control. (g) Schematic representation of the V1, V2 and V3 forms of MELK. The shaded boxes indicate the catalytic domain (amino acids 11 to 263 of the V1 protein). The KA1 domain is the kinase-associated domain in the carboxy-terminal region.
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    Image Search Results


    Expression and distribution of MELK in human normal tissues and breast cancer cell lines. (a) Expression of MELK in 12 breast cancer specimens (case number; 42, 102, 247, 252, 302, 473, 478, 502, 552, 646, 769 and 779) by semi-quantitative RT-PCR. GAPDH served as a quantitative internal control. (b) Multiple tissue Northern blot analysis demonstrated that an approximately 2.7 kb MELK transcript was detected in the testis, thymus and small intestine. PBL, peripheral blood leukocytes. (c) Breast cancer cell line Northern blot analysis revealed that approximately 2.4 to 2.7 kb MELK variants were specifically expressed in breast cancer cell lines, but not in normal vital organs. (d) Schematic representation of three variant transcripts identified by cDNA library screening (see Materials and methods). White boxes indicate a coding region and black boxes indicate a non-coding region. Black and grey triangles indicate initiation codons, and white triangles indicate stop codons. Exon numbers are shown above each box. (e) In vitro translation assay of each variant isolated from cDNA library screening. The number within parentheses represents the predicted molecular weight (kDa) of each variant protein. (f) Expression of MELK proteins in eight breast cancer cell lines as well as human mammary epithelial cells (HMECs) shown by western blot analysis with an anti-MELK antibody. β-Actin served as a control. (g) Schematic representation of the V1, V2 and V3 forms of MELK. The shaded boxes indicate the catalytic domain (amino acids 11 to 263 of the V1 protein). The KA1 domain is the kinase-associated domain in the carboxy-terminal region.

    Journal: Breast Cancer Research

    Article Title: Involvement of maternal embryonic leucine zipper kinase (MELK) in mammary carcinogenesis through interaction with Bcl-G, a pro-apoptotic member of the Bcl-2 family

    doi: 10.1186/bcr1650

    Figure Lengend Snippet: Expression and distribution of MELK in human normal tissues and breast cancer cell lines. (a) Expression of MELK in 12 breast cancer specimens (case number; 42, 102, 247, 252, 302, 473, 478, 502, 552, 646, 769 and 779) by semi-quantitative RT-PCR. GAPDH served as a quantitative internal control. (b) Multiple tissue Northern blot analysis demonstrated that an approximately 2.7 kb MELK transcript was detected in the testis, thymus and small intestine. PBL, peripheral blood leukocytes. (c) Breast cancer cell line Northern blot analysis revealed that approximately 2.4 to 2.7 kb MELK variants were specifically expressed in breast cancer cell lines, but not in normal vital organs. (d) Schematic representation of three variant transcripts identified by cDNA library screening (see Materials and methods). White boxes indicate a coding region and black boxes indicate a non-coding region. Black and grey triangles indicate initiation codons, and white triangles indicate stop codons. Exon numbers are shown above each box. (e) In vitro translation assay of each variant isolated from cDNA library screening. The number within parentheses represents the predicted molecular weight (kDa) of each variant protein. (f) Expression of MELK proteins in eight breast cancer cell lines as well as human mammary epithelial cells (HMECs) shown by western blot analysis with an anti-MELK antibody. β-Actin served as a control. (g) Schematic representation of the V1, V2 and V3 forms of MELK. The shaded boxes indicate the catalytic domain (amino acids 11 to 263 of the V1 protein). The KA1 domain is the kinase-associated domain in the carboxy-terminal region.

    Article Snippet: Breast cancer Northern blots and human multiple-tissue Northern blots (Takara Clontech) were hybridized with [α 32 P]-dCTP-labeled PCR products of MELK cDNA prepared by RT-PCR (see below).

    Techniques: Expressing, Quantitative RT-PCR, Northern Blot, Variant Assay, cDNA Library Assay, In Vitro, Isolation, Molecular Weight, Western Blot

    A, Western blot of total lysates using anti-NFRKB reveals a double band at the expected size. The specificity was attested by the loss of the binding upon preincubation of antibody with synthetic peptides corresponding to positions 543–557 and 1091–1106 within the primary structure of NFRKB. These data are representative of three independent experiments. B, Northern blot analysis of NFRKB. Multiple tissue-Northern blot (Clontech Laboratories, Inc.) was hybridized with a full-length-probe and exposed to Phosphoimager Storm for 24 h. This experiment was repeated once.

    Journal: PLoS ONE

    Article Title: Upregulation of Nuclear Factor-Related Kappa B Suggests a Disorder of Transcriptional Regulation in Minimal Change Nephrotic Syndrome

    doi: 10.1371/journal.pone.0030523

    Figure Lengend Snippet: A, Western blot of total lysates using anti-NFRKB reveals a double band at the expected size. The specificity was attested by the loss of the binding upon preincubation of antibody with synthetic peptides corresponding to positions 543–557 and 1091–1106 within the primary structure of NFRKB. These data are representative of three independent experiments. B, Northern blot analysis of NFRKB. Multiple tissue-Northern blot (Clontech Laboratories, Inc.) was hybridized with a full-length-probe and exposed to Phosphoimager Storm for 24 h. This experiment was repeated once.

    Article Snippet: Human multiple-tissue Northern blot (CLONTECH Laboratories, Inc.) was hybridized with a cDNA probe corresponding to the full-length coding sequence.

    Techniques: Western Blot, Binding Assay, Northern Blot